A synonymous single nucleotide polymorphism (SNP) is a substitution of a single base that does not modify the primary amino acid sequence but could influence protein function. In patients with brain tumors, the incidence of the silent SNP IDH 1 105GGT (rs11554137) is three times higher than the normal population.
Our aim was to investigate the prognostic role of the IDH 1 105GGT SNP. We selected only patients with diagnosis of IDH grade II or III mutated glioma. Additional inclusion criteria were complete clinical data and adequate tumor samples for IDH 1 or 2 sequencing.
71 patients with grade II and III IDH-mutated glioma have been evaluated. Nine of 71 patients (12.7 %) presented the SNP . Patients with SNP had a longer Progression Free Survival (PFS - 47.3 months vs Not reached; p?=?0.015). The SNP (HR 0.240; 95 %CI 0.074-0.784, p?=?0.018) was confirmed as an independent prognostic factors in multivariate analysis.
Patients with IDH1 or 2 mutated grade II and III glioma presenting the SNPhad longer PFS regardless adjuvant treatment received and extension of primary surgery. A validation is warranted to confirm our preliminary results.
Patients with IDH1 or 2 mutated grade II and III glioma presenting the SNP105GGT had longer PFS regardless adjuvant treatment received and extension of primary surgery. A validation is warranted to confirm our preliminary results.anaplastic lymphoma kinase (ALK) overexpression and gene alterations have been detected in several malignancies, with prognostic and therapeutic implications. However, few studies investigated the correlation between ALK altered expression and prognosis in patients with glioblastoma (GBM).
We performed an evaluation of ALK overexpression and structural/quantitative chromosome alterations through immune-histochemical assay (IHC with D5F3 antibody) and fluorescent in situ hybridization (FISH) in patients with isocitrate dehydrogenase (IDH) wild type (wt) GBM. Assuming an ALK overexpression in 20 % of patients we planned a sample of 44 patients to achieve a probability of 90 % to include from 10 % to 30 % of patients with ALK alterations.
We evaluated 44 patients with IDH wt GBM, treated in our institution and dead due to GBM progression in 2017. ALK overexpression obtained by a composed score (the product of IHC intensity staining and rate of positive cells) was observed in 19 (43 %) patients. FISH analysis showed that 11 patients (25 %) had gene deletion, 2 patients (4.5 %) had monosomy and one patient (2.3 %) presented polysomy. Only one patient (2.3 %) demonstrated ALK rearrangement. There was no statistical difference in median OS between patients with ALK-positive (mOS?=?18.9 months) and ALK-negative IHC (mOS?=?18.0 months).
We identified some rare previously unreported alterations of ALK gene in patients with IDH wt GBM. In these patients, the ALK overexpression does not influences survival.
We identified some rare previously unreported alterations of ALK gene in patients with IDH wt GBM. In these patients, the ALK overexpression does not influences survival.Gastric cancer is a malignant tumor originating from the gastric mucosal epithelium, with no obvious symptoms at the early stage. The dopamine transporter gene (SLC6A3) is involved in the metabolism of dopamine and catecholamine and is a potential gene for Parkinson's disease and alcoholism. But the role of SLC6A3 in gastric cancer is still unknown. The aim of our study is to investigate the potential diagnostic value of SLC6A3 on gastric cancer.
Quantitative real-time PCR (RT-qPCR) was used to detect the expression of SLC6A3 in clinical samples and cells. A total of 246 samples were enrolled in this study (26 pairs of tissue samples; Serum of 113 patients with gastric cancer, 51 polyps patients and 56 healthy controls). The diagnostic value of SLC6A3 was evaluated by the ROC curve and analyzed the changes of SLC6A3 expression before and after surgery. The prognostic value, interacting proteins and related pathways of SLC6A3 were evaluated by TCGA analysis in UALCAN database (http//ualcan.path.uab.edu/).
The expression level of SLC6A3 in gastric cancer was significantly higher than that in controls. Further, the proportion under the ROC curve (AUC) for SLC6A3, CEA and CA19-9 was 0.818 (95 % confidence interval [CI] 0.754 to 0.883, P &lt; 0.001), and the expression level of SLC6A3 in the serum of patients with gastric cancer decreased significantly after surgery (P &lt; 0.001). Bioinformatic enrichment analysis of SLC6A3 displayed the relevant metabolic pathways involved in its interacting proteins.
SLC6A3 is involved in the occurrence and development of gastric cancer and can be used as a potential diagnostic indicator for gastric cancer.
SLC6A3 is involved in the occurrence and development of gastric cancer and can be used as a potential diagnostic indicator for gastric cancer.Reactions of biomass conversions are of great importance in fine chemistry for substantial development. While numerous studies have been performed to search for functional materials to catalyze biomass conversions, a robust and high-throughput analytical method is rather limited, which may hamper further integration and automation of the reactions. Here we propose an automatic and sequential method for the investigation of glucose conversion. By combining sequential sample injection and high-speed capillary electrophoresis (HSCE) techniques, we can monitor the glucose conversion from the beginning toward the end with a good temporal resolution. The HSCE assays are performed using short capillaries (effective length of 10 cm, i.d./o.d. https://www.selleckchem.com/products/d34-919.html of 50 μm/365 μm), and the analytes are separated at an electric field of 467 V/cm and are detected by UV-absorption at 200 nm with mixed 0.2 mM CTAB, 10 mM borate, 20 mM sorbic acid (pH 12.2) as the background electrolyte. All compounds involved in the reaction, including all products (fructose, 5-hydroxymethylfurfural, formic acid and levulinic acid) and the remaining substrate glucose, are efficiently separated and simultaneously detected from just one analysis with a temporal resolution of one minute. The method exhibits high-resolution separation, a wide linear range with limit-of-detection down to μg/mL-level, as well as excellent repeatability in sequential analysis. It is indicated that the proposed method is of great value in the analysis of complicated biomass conversion and could be potentially applied in various catalytic chemical reactions.